In spite of its higher cost, agar is still used as most frequent gelling
agent in commercialisation of plant tissue culture. The potential of tissue
culture medium gelled with starch of two different varieties to support
seed germination of Glycine max L. and morphogenetic response of various
explants (shoot tip, leaf and root) was studied. Explants were obtained
from one week old Glycine max L. plants raised in vitro. By analysing various
responses, it was found that although there was no significant difference
between the two gelling agents (agar and starch) in response to seed germination,
yet the starch gelled medium was not found appropriate to replace agar for
morphogenetic responses of various explants in culture.
Key words : Gelling agent, starch, agar, morphogenetic response.